January 23 - January 23
Characterizing liposome formation, structure, and stability with complementary techniques
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Liposomes have been used in drug discovery and drug delivery for some time, and the biophysical characterization of these systems and their payloads is critical to understanding and optimizing their fabrication and function. This study looks at optimal conditions for extruding liposomes as well as their stability under different conditions.
Our aim is to further educate the public about the intricacies of liposome formation and characterization as measured by Nanoparticle Tracking Analysis (NTA) from the NanoSight product range, Dynamic and Electrophoretic Light Scattering (DLS/ELS) from the Zetasizer product range, and Small-angle and Wide-angle X-ray scattering (SAXS/WAXS) from the X-ray analytical product range within Malvern Panalytical.
A broad range of characterization information and combination of both NanoSight and Zetasizer systems helped further optimize fabrication and understand the function of liposomes as well as labeling efficiency of fluorescent lipids. NTA through NanoSight provided number-based high resolution sizing, accurate distribution profiles, concentration (particles/mL), and fluorescence measurements. DLS provided excellent reproducibility, mean size and PDI measurements over a broad range and non-invasive trend analysis. ELS provided zeta potential as a functionality and stability metric of particles. SAXS allowed to investigate the bilayer stacking in MLVs and from WAXS data the order of the alkyl chains (gel phase vs. liquid phase) could be studied as a function of sample temperature.
Time: 10:30 - 11:30 (GMT-05:00) Eastern [US & Canada]
About our speakers:
Ragy Ragheb is a Technical Specialist at Malvern Panalytical specializing in the NanoSight and Zetasizer product ranges. Ragy received his Ph.D. in Macromolecular Science and Engineering from Virginia Polytechnic Institute and State University and completed his post-doctoral work in Biomedical Engineering at Yale University. His research focused on the fabrication and characterization of several synthetic and metal-based nanoparticle systems for theranostic and drug delivery applications.
Joerg Bolze studied Chemistry at the University of Karlsruhe in Germany. In 1997 he received a doctorate for his thesis about the structural characterization of colloidal systems with small-angle X-ray scattering. He continued his research as a postdoctor at synchrotron radiation facilities in Japan and Korea. In 2006 Joerg joined PANalytical as an application specialist for small-angle X-ray scattering. In 2012 he was appointed as Product Marketing manager Nanomaterials. Over the years he contributed significantly to the development of Malvern PANalytical's small- and wide angle X-ray scattering solutions.
- Characterizing liposome formation, structure, and stability with complementary techniques
- 23 January 2018, 10:30 - 23 January 2018, 11:30
- UTC-06:00 US/Mountain