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Transmission diffraction. Measurement and unit cell refinement of lysozyme

Transmission experiments were performed on a hen egg-white lysozyme sample. The high angular resolution of the data enabled cell searching, indexing and unit cell refinement. The lattice parameters determined were in good agreement with the data obtained from single crystal measurements.

Diffraction experiments on protein samples have traditionally been relegated to single crystal diffraction systems for determining structure, or beam lines for extracting powder patterns from very small quantities of poorly diffracting material.

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